endobj 2. BrdU kann von der Zelle aufgenommen werden und in phosphorylierter Form anstelle des Nukleotids … 1 0 obj Invitrogen Anti-BrdU monoclonal antibody (such as Cat. 5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo. For in vivo labeling by IP injection, see In vivo labeling of mouse cells with BrdU (page 21). Print this protocol BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal. A 10 mg/ml solution of BrdU in sterile 1X DPBS is provided for in vivo use. DNase. Inject n mice with 1.6 mg BrdU in 200 µl i.p. Es entsteht durch Verbindung von Desoxyribose mit der Nucleobase 5-Bromuracil. .��gIJ�������v���6L��$�'�r�զ��ݢb���_;/������|䓿*��M��_��JL�*9��B���D��˻{^���bGF姩ظ��펤������a�;���� �^��3�z*�۝Q��{` p��& <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 595.32 841.92] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> DNase can be purchased separately from Sigma (Catalog No. Other Notes This product can be found as purified product that was produced using cell culture hybridoma product. EdU (5-ethynyl-2’-deoxyuridin e) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. <> BrdU labeling can be performed in vitrofor cell lines and primary cell cultures, orin vivofor labeling cells within a living animal. B. BrdU detection – Day 1 3. (1:3.33 dilution of 20 mg/ml stock). hޔR]k�0�+zk�Pk%�G \�k0���� ���۫�$2���*�}ɵY#�J;;#���Lʄ�0f2��� �����LRt�s��[}XC ̍D�@H�fn���]}&�� ]FU�]+�h��=�q��0�jU�0�XVK©�!��㈉���W���Қ�����z둨������H�$�ݵ��uN�p�͔9S�SY��(e

We keep those stocks in the -20 deg C freezer. This methodology allows detection of endothelial cells in the S phase at the light microscopic level but, unlike autoradiography, there is no 3- to 4-week exposure period. ��T�v�.ڮew�l��=v��>�{k���6+�E�VT��VT�-�o ���5�6?��Y��6R�$R:�o�UtH�Q`p��r6�c�"�;_���Y�n�Uz�S���G���F��,��n�g�Gp6 2 0 obj hޔR]k�0�+zk�Pk%�G \�k0���� ���۫�$2���*�}ɵY#�J;;#���Lʄ�0f2��� �����LRt�s��[}XC ̍D�@H�fn���]}&�� ]FU�]+�h��=�q��0�jU�0�XVK©�!��㈉���W���Қ�����z둨������H�$�ݵ��uN�p�͔9S�SY��(e BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. x��XIoT9������)n� aFBb ����҄H$̈́�0�~����t�� ��W��.�kq{��vw�i=���ǫ���z���`V�ۯW��|ݬ^�/�nֻ�������v(�}����>yž��������K&���kł��`�8��h/�yv� g6F3�����2i��ڷ/��G��#;�c�x�Y.~���e����v��r��GU�/.���Mh�����&��-{��[A�+q��"�0QP�h�#�eIn�5�PM˅�\�X�s-mJ(͌�*\8FC� t�9! d�r5{����.��u��ƺ%B�HI�"�*aj���{� �U�,&�2B�Q��~���Ȋ\� z�Dr�s�S�A�n�C�L�Qɦ�G΍����1y�]���!��N*�U/� 5��#:X�1p�uE�^B��U��,Xl�I�'en&;o�`���"�Ѩ��(�A֒P����-�w��͖���� �}Y*B���X�)*���7k��i��N�Fh�! stream �Q�F�=%9�z��v/��w?h��q&�k�. The application of monoclonal antibodies which react specifically with BrdU for detection of DNA replication in lymphoid cells and other normal or pathological preparations, following in vivo or in vitro BrdU labeling, is extensively documented in the biomedical literature. Bromodeoxyuridine (BrdU) is a thymidine analog to which monoclonal antibodies have been developed.

10-12 hrs later inject same mice with 1.2 mg BrdU in 200 µl i.p. vivo for labeling cells within a living animal. with 100-200 µl (1-2 mg) of BrdU solution. 237 0 obj <>stream �0"Rq*��4RA�~�Elψ0I After BrdU labeling, an additional DNA hydrolysis step (sometimes referred to as a DNA denaturing step) may be required after fixation and permeabilization to allow the anti-BrdU antibody access to the BrdU within the DNA . ��Y]�JC�mFr� BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies. Desoxyuridin.