EdU-594 100 assays. EdU is a thymidine analog, and its attached alkyne group is rare in natural compounds. Analysis of the effects of drugs on cell proliferation and NF-KB signaling pathway in combination with P65 antibody and EdU. It can infiltrate thymine (T) into the DNA molecule being synthesized during DNA replication. Golgi (in green) is labeled with Alexa Fluor® 488 conjugate of lectin HPA, and peroxisomes (in orange) are labeled with a rabbit primary followed by Alexa Fluor® 555 donkey anti–rabbit IgG antibody. Based on the specific reaction of Apollo® fluorescent dyes with EdU, DNA replication activity can be directly and accurately detected. <> The EdU cell proliferation assay has been applied to many different cell types and organism. - Find MSDS or SDS, a COA, data sheets and more information. Muntjac cells pulsed with EdU, fixed and permed. HeLa cells (4 x 10EdU staining of proliferating cells. Submitted Mar 31 2020 ���� JFIF ` ` �� C stream

Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. In this method, the thymidine analog EdU (5-ethynyl-2´-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle.

10Dot plot of EdU-647 staining (Y-axis, 647) vs FSC. Proliferation, however, is rarely assayed in isolation; other cell function probes are often used in concert with proliferation assays to provide a more … endobj

Introduction and product description: The detection of cell proliferation is of utmost importance for assessing cell health, determining genotoxicity or evaluating anticancer drugs. 3 0 obj EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is … 10EdU staining of proliferating cells. Selected product Cell Proliferation Kits (EdU-FM). Cell-division cycle is a series of events resulting in two daughter cells containing replicas of DNA from the original DNA molecule. <> ",#(7),01444'9=82

endobj The reaction of … 5 0 obj Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry (See a list of the products featured in this article.) The Apollo®643 dye detection result was in gray-scale value, and the picture was rendered in red.Figure 8. <>

<> Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye | Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody.Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cellsProliferating cells labeled in vivo with the Click-iT® EdU assay.Multicolor imaging with Click-iT® EdU. The iFluor 647 dye (Ex/Em: 649/664 nm) has spectral properties almost identical to those of Cy5EdU staining protocol summary (wash cells between each step):EdU staining can also be combined with antibody staining or cell staining with other fluorescent dyes.This kit provides enough reagents to perform 50 flow cytometry tests or 50 microscopy tests (for 18 x 18 mm coverslips) or 200 microscopy tests (adapted for 96-well plate format).Previously called EdU Proliferation Assay Kit (iFluor 647).The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. We use cookies to make our site as useful as possible. %���� Your browser does not have JavaScript enabled and some parts of this website will not work without it.For the best experience on the Abcam website please upgrade to a modern browser such as EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog that is an alternative to BrdU, is also used in DNA proliferation assays that are simpler and faster than the BrdU assay. EdU Cell Proliferation Kit The EdU-Click Kitcontains chemicals to perform 100 reactions (500 µL each). <> Product Description. The main advantages of EdU staining over using BrdU are:Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.Dot plot of EdU-647 staining (Y-axis, 647) vs FSC.