Papers

Methodological considerations for detecting neurogenesis in the adult brain. BrdU labeling is currently the most used technique for studying adult neurogenesis in situ.

JabRef All of which have profound consequences on neurogenesis. Darmer D, Hauser F, Nothacker HP, Bosch TC, Williamson M, Grimmelikhuijzen CJ (1998) Three different prohormones yield a variety of Hydra-RFamide (Arg-Phe- NH2) neuropeptides in Hydra magnipapillata. Development 121:1205–1216Chera S, Ghila L, Wenger Y, Galliot B (2011) Injury-induced activation of the MAPK/CREB pathway triggers apoptosis-induced compensatory proliferation in hydra head regeneration. Spend your experimental break wisely and get some science on the go in less than 15 minutes.

Cell Motil Cytoskeleton 55(2):77–96Charles P et al (2002) Re-expression of PSA-NCAM by demyelinated axons: an inhibitor of remyelination in multiple sclerosis? This protocol is to provide the experimenter a method to label proliferating cells in the adult mouse brain and identify those cells by immunohistochemistry techniques frequently used when assessing adult neurogenesis.

Introduction. In: Breidbach O, Kutsch W (eds) The nervous systems of invertebrates: an evolutionary and comparative approach. During the BrdU assay, BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies.

BrdU immunohistochemistry for studying adult neurogenesis: paradigms, pitfalls, limitations, and validation. Furthermore, to examine adult neurogenesis, we labeled proliferating cells by BrdU and quantified the BrdU/NeuN double-positive cells after 4 weeks (Fig. In summary, the presented studies tell a cautionary tale that relates to interpreting results derived from the use of BrdU in neurogenesis studies, and emphasize the importance of including controls in your experiments to account for off-target effects. Arch Phys Med Rehabil 86(10):1901–1909Coronado VG et al (2005) The CDC traumatic brain injury surveillance system: characteristics of persons aged 65 years and older hospitalized with a TBI.

Development 125:1637–1645Gauchat D, Escriva H, Miljkovic-Licina M, Chera S, Langlois MC, Begue A, Laudet V, Galliot B (2004) The orphan COUP-TF nuclear receptors are markers for neurogenesis from cnidarians to vertebrates.

Trends Cogn Sci 9(3):136–143Cicerone KD et al (2005) Evidence-based cognitive rehabilitation: updated review of the literature from 1998 through 2002. Neurosci Biobehav Rev 28(4):365–378Kuhn HG, Dickinson-Anson H, Gage FH (1996) Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. Other studies on embryonic stem cell derived neural stem cells (NSCs) showed that exposure to BrdU resulted in loss of global DNA methylation (Schneider and d’Adda di Fagagna 2012).

Conversion of neurons to expression of a vasopressin-like immunoreactivity depends on axial location. Therefore, BrdU labeling without the use of additional markers is inappropriate.

Curr Biol 18:1156–1161Teragawa CK, Bode HR (1995) Migrating interstitial cells differentiate into neurons in hydra. Despite these findings, early studies on adult neurogenesis did not consider these possible side effects of BrdU. J Exp Zool 132Gierer A, Berking S, Bode H, David CN, Flick K, Hansmann G, Schaller H, Trenkner E (1972) Regeneration of hydra from reaggregated cells. I. Mendeley Differentiation 68(2–3):141–152Ignatova TN et al (2002) Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro. Nat Rev Neurosci 2(4):287–293Doetsch F, Garcia-Verdugo JM, Alvarez-Buylla A (1997) Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. J Neurosci 24(42):9232–9239Herrup K, Yang Y (2007) Cell cycle regulation in the postmitotic neuron: oxymoron or new biology? Novartis Found Symp 231:220–235; discussion 235–241, 302–306Rakic P (1972) Mode of cell migration to the superficial layers of fetal monkey neocortex.

8a,c,d, arrow). Zotero J Neurotrauma 22(2):252–265Fujimoto ST et al (2004) Motor and cognitive function evaluation following experimental traumatic brain injury. Neurosci Lett 369(1):24–27Florenes VA et al (1994) Expression of the neuroectodermal intermediate filament nestin in human melanomas. Brain Res 118(2):281–291Yu WH (1979) Restitution of bromodeoxyuridine-induced changes in cerebellar development. Currently, there is no effective treatment for this disorder.